Subacute Necrotizing Encephalomyelopathy and MT-CO1
Gene Motifs and Protein Domains
A myriad of online sequencing databases are available to the public in order to find the motifs and domains of genes and proteins respectively. Motifs are a pattern that are widespread and has, or are deemed to have a biological significance. Domains are parts of a protein sequence and structure that can evolve, function, and exist independently of the rest of the protein chain.
The MT-CO1 Motifs were analyzed by numerous online databases. The databases were selected out of a large list due to their ease of use and availability. First the restriction endonuclease sites of the gene were depicted. Motif was then used to find biologically significant motifs throughout the gene. Finally MEME and the YMF motif finder were used to find repeating patterns within the gene. These genes were blasted with TOMTOM and GLAM2 to determine if these repeats had any biological significance. None were found.
The Cytochrome c Oxidase Subunit 1 Domains were then analyzed. The databases were selected out of a large list due to their ease of use and availability. The initial analysis showed the codon bias for Homo sapiens from Gene Infinity Codon Usage, as for each organism the codon bias is different. Next SMART and PROSITE were used to find functional domains within the protein yielding transmembrane and copper binding domains respectively. Finally, ProDom was used determine a myriad of other domains, each with their own identifying reference number.
None of the motifs or domains was surprising based on the function of the protein.
The MT-CO1 Motifs were analyzed by numerous online databases. The databases were selected out of a large list due to their ease of use and availability. First the restriction endonuclease sites of the gene were depicted. Motif was then used to find biologically significant motifs throughout the gene. Finally MEME and the YMF motif finder were used to find repeating patterns within the gene. These genes were blasted with TOMTOM and GLAM2 to determine if these repeats had any biological significance. None were found.
The Cytochrome c Oxidase Subunit 1 Domains were then analyzed. The databases were selected out of a large list due to their ease of use and availability. The initial analysis showed the codon bias for Homo sapiens from Gene Infinity Codon Usage, as for each organism the codon bias is different. Next SMART and PROSITE were used to find functional domains within the protein yielding transmembrane and copper binding domains respectively. Finally, ProDom was used determine a myriad of other domains, each with their own identifying reference number.
None of the motifs or domains was surprising based on the function of the protein.
