Subacute Necrotizing Encephalomyelopathy and MT-CO1
RNAi
Ribonucleic Acid Interference (RNAi) is an emerging area of study, in which cells are able to protect themselves against foreign RNA. The foreign DNA is recognized by a RISC complex within cells. This complex activates a DICER molecule to degrade the foreign RNA and any RNA that has a similar sequence [1].
This appears to be ancestral defense mechanism against viruses, however is currently being used as research in a myriad of human health issues. It use in the study of SNEM would allow us to create knockout organisms for Subunit 1 of Cytochrome c Oxidase.
This appears to be ancestral defense mechanism against viruses, however is currently being used as research in a myriad of human health issues. It use in the study of SNEM would allow us to create knockout organisms for Subunit 1 of Cytochrome c Oxidase.
RNAi in Drosophila Melanogaster
Drosophila. Picture obtained from Wikipedia
The gene that encodes for Cytochrome c Oxidase subunit 1 in Drosophila melanogaster is mt:Co1 (192469) [2,3,4]. However no RNAi screens have been done for this gene.
RNAi in Caenorhabditis elegans
C. elegans. Picture obtained from Wikipedia
The gene that encodes for cytochrome c oxidase in Caenorhabditis elegans (2562700) has not been analyzed in any of the suggested online databases [5,6,7]. Therefore no RNAi screens have been completed.
References
[1] Hannon, G. (2005, July). Nova Science Now. (Public Broadcasting Service) Retrieved April 2010, from RNAi: http://www.pbs.org/wgbh/nova/sciencenow/3210/02.html
[2] FLIGHT (2010) Flight Version 2.0. Retrieved April 2010, from mt-CoI: http://flight.licr.org/search/return_gene_results.jsp?gene_id=FBgn0013674&taxid=7227&return=6
[3] Fly Base. (2010, April 23). Fly Base. Retrieved April 2010, from mt:Co1: http://flybase.org/reports/FBgn0013674.html
[4] Harvard Medical School. (n.d.). Drosophila RNAi Screening Center. Retrieved April 2010, from mt:Co1: http://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl
[5] California Institute of Technology, Cold Spring Harbor Laboratory, Washington University at St. Louis, and the Welcome Trust Sanger Institute. (2010). WormBase. Retrieved April 2010, from: http://www.wormbase.org/
[6] Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). (2010). Worm Protein DataBase. Retrieved April 2010, from http://worm-srv1.mpi-cbg.de/dbscreen/index.html
[7] PhenoBank. (2010). PhenoBank DataBase. Retrieved April 2010, from http://www.worm.mpi-cbg.de/phenobank2/cgi-bin/MenuPage.py
[2] FLIGHT (2010) Flight Version 2.0. Retrieved April 2010, from mt-CoI: http://flight.licr.org/search/return_gene_results.jsp?gene_id=FBgn0013674&taxid=7227&return=6
[3] Fly Base. (2010, April 23). Fly Base. Retrieved April 2010, from mt:Co1: http://flybase.org/reports/FBgn0013674.html
[4] Harvard Medical School. (n.d.). Drosophila RNAi Screening Center. Retrieved April 2010, from mt:Co1: http://www.flyrnai.org/cgi-bin/DRSC_gene_lookup.pl
[5] California Institute of Technology, Cold Spring Harbor Laboratory, Washington University at St. Louis, and the Welcome Trust Sanger Institute. (2010). WormBase. Retrieved April 2010, from: http://www.wormbase.org/
[6] Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). (2010). Worm Protein DataBase. Retrieved April 2010, from http://worm-srv1.mpi-cbg.de/dbscreen/index.html
[7] PhenoBank. (2010). PhenoBank DataBase. Retrieved April 2010, from http://www.worm.mpi-cbg.de/phenobank2/cgi-bin/MenuPage.py
