Gene Homology
The gene sequence of MT-CO1 (human) was blasted to find the the closest 17 orthologs
[1]. The orthologs were then collected and aligned using various free online alignment programs. This analysis was then compiled into phylogenetic trees.
Each alignment program yielded similar results, however based on the algorithms, values of homology varied slightly.
The FASTA sequences of all orthologs are compiled in the text file below.
The species names of the gene orthologs are used on the alignment. The common name is matched below.
Species
Arabadopsis thaliana
Oryza sativa
Caenorhabditis elegans
Drosophila melanogaster
Anopheles gambiae
Gallus gallus
Danio rerio
Canis lupus familiaris
Homo sapiens
Pan troglodytes
Bos taurus
Mus musculus
Rattus norvegicus
Saccharomyces cerevisiae
Kluyveromyces lactis
Ashbya gossypii
Schizosaccharomyces pombe
Plasmodium falciparum
ClustalW Alignment
The alignment using
ClustalW 2.0.3 is compiled into the text file below
[2]. The default settings were used during the alignment. The phylogenetic tree is shown below.
mt-co1_clustalw_alignment.rtf |
File Size: | 324 kb |
File Type: | rtf |
Download File
MUSCLE Alignment
The alignment using
MUSCLE 3.7 is compiled into the text file below
[3]. The default settings were used during the alignment. The phylogenetic tree is shown below.
mt-co1_muscle_alignment.rtf |
File Size: | 517 kb |
File Type: | rtf |
Download File
T-Coffee Alignment
The alignment using
T-Coffee 6.85 is compiled into the text file below
[4]. The default settings were used during the alignment. The phylogenetic tree is shown below.
mt-co1_t-coffee_alignment.rtf |
File Size: | 163 kb |
File Type: | rtf |
Download File
GeneBee Alignment
The alignment using
GeneBee is compiled into the word file below
[5]. The default settings were used during the alignment.
mt-co1_genebee_alignment.rtf |
File Size: | 270 kb |
File Type: | rtf |
Download File
References
[1] National Center for Biotechnology Information. Basic Local Assignment Search Tool. Retrieved March 23, 2010, from Blastn:
http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch&SHOW_DEFAULTS=on&LINK_LOC=blasthome[2] GForge Project. Methodes et Algorithms pour la Bio-Informatique. Retrieved March 23, 2010, from
ClustalW 2.0.3:
http://www.phylogeny.fr/version2_cgi/one_task.cgi?task_type=clustalw[3] GForge Project. Methodes et Algorithms pour la Bio-Informatique. Retrieved March 23, 2010, from
MUSCLE 3.7:
http://www.phylogeny.fr/version2_cgi/one_task.cgi?task_type=muscle[4] GForge Project. Methodes et Algorithms pour la Bio-Informatique. Retrieved March 23, 2010, from
T-Coffee 6.85:
http://www.phylogeny.fr/version2_cgi/one_task.cgi?task_type=tcoffee[5] GeneBee Group. GeneBee - Molecular Biology Server. Retrieved March 23, 2010, from
Alibee - Multiple Alignment:
http://www.genebee.msu.su/services/malign_reduced.htmlReferences specific to phylogenetic trees (other than Genebee):1. Dereeper A.*, Guignon V.*, Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.F., Guindon S., Lefort V., Lescot M., Claverie J.M., Gascuel O.
Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9. Epub 2008 Apr 19. (
PubMed)
*: joint first authors 2. Edgar RC.
MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, Mar 19;32(5):1792-7. (
PubMed)
3. Castresana J.
Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000, Apr;17(4):540-52. (
PubMed)
4. Huelsenbeck JP., Ronquist F.
MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001, Aug;17(8):754-5. (
PubMed)
5. Chevenet F., Brun C., Banuls AL., Jacq B., Chisten R.
TreeDyn: towards dynamic graphics and annotations for analyses of trees. BMC Bioinformatics. 2006, Oct 10;7:439. (
PubMed)